| 产品编号 | bs-47146P |
| 英文名称 | Recombinant human LIGHT (Trimer) protein, N-His-Flag (HEK293) |
| 中文名称 | 重组人LIGHT (Trimer)蛋白 |
| 别 名 | Tumor necrosis factor ligand superfamily member 14; CD 258; CD258; CD258 antigen; Delta transmembrane LIGHT; Herpes virus entry mediator ligand; Herpesvirus entry mediator A; Herpesvirus entry mediator ligand; herpesvirus entry mediator-ligand; HVEM L; HVEM-L; HVEML; Ligand for herpesvirus entry mediator; LIGHT; LTg; soluble form; TNF14; TNFSF 14; TNFSF14 protein; TR 2; TR2; Tumor necrosis factor (ligand) superfamily, member 14; Tumor necrosis factor ligand superfamily, member 14; Tumor necrosis factor receptor like 2; Tumor necrosis factor superfamily member LIGHT; TNF14_HUMAN; Recombinant human LIGHT (Trimer) protein, N-His-Flag (HEK293) |
| 理论分子量 | 52.4kDa |
| 检测分子量 | Due to glycosylation, the protein migrates to 52-140 kDa based on Tris-Bis PAGE result. |
| 性 状 | Lyophilized powder |
| 浓 度 | ＞0.5 mg/ml |
| 物 种 | Human |
| 序 列 | 74-240/240 |
| 纯 度 | >95% as determined by Tris-Bis PAGE; >95% as determined by SEC-HPLC |
| 纯化方法 | AC |
| 内毒素 | <1.0 EU/μg as determined by LAL |
| 表达系统 | HEK293 cell |
| 活性 | Not tested |
| 标签 | N-His-Flag |
| 缓 冲 液 | Lyophilized from 0.22 um filtered solution in PBS (pH=7.4) with 5 mM DTT. Normally 5% trehalose is added as protectant before Lyophilization. |
| 保存条件 | Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 | |
| 产品图片 |
Immobilized Human HVEM(Cat.HVE-HM211) at 0.5ug/ml(100ul/Well).Dose response curve for Human Trimer LIGHT, with the EC50 of 0.24ug/ml determined by ELISA.
The purity of LIGHT(Cat.LGT-HM132) is greater than 95% as determined by SEC-HPLC.
Recombinant LIGHT on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
