| 产品编号 | bs-47157P-Biotin |
| 英文名称 | Recombinant human Mesothelin, C-His-Avi (HEK293) / Biotin |
| 中文名称 | 生物素标记重组人Mesothelin蛋白 |
| 别 名 | MSLN; MES; CAK 1; CAK1; CAK1 antigen; Megakaryocyte potentiating factor; Mesothelin isoform 1 precursor; MPF; Pre pro megakaryocyte potentiating factor; SMR; SMRP; Soluble MPF mesothelin related protein; MSLN_HUMAN; Recombinant human Mesothelin, C-His-Avi (HEK293) / Biotin |
| 理论分子量 | 35.2kDa |
| 检测分子量 | Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result. |
| 性 状 | Lyophilized powder |
| 浓 度 | ＞0.5 mg/ml |
| 物 种 | Human |
| 序 列 | 296-580/630 |
| 纯 度 | >95% as determined by Tris-Bis PAGE; >95% as determined by SEC-HPLC |
| 纯化方法 | AC |
| 内毒素 | <1.0 EU/μg as determined by LAL |
| 表达系统 | HEK293 cell |
| 活性 | Not tested |
| 标签 | C-His-Avi |
| 缓 冲 液 | Lyophilized from 0.22 um filtered solution in PBS (pH=7.4) with 5 mM DTT. Normally 5% trehalose is added as protectant before Lyophilization. |
| 保存条件 | Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 | |
| 产品图片 |
Immobilized Biotinylated Human MSLN,His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Anti-MSLN Ab.with the EC50 of 3.7ng/ml determined by ELISA.
The purity of Biotinylated Human Mesothelin was greater than 95% as determined by SEC-HPLC.
Biotinylated Human Mesothelin on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
