| 产品编号 | bs-47173P |
| 英文名称 | Recombinant human PD-1 protein, C-His (HEK293) |
| 中文名称 | 重组人PD-1蛋白 |
| 别 名 | Programmed cell death protein 1; CD279; CD279 antigen; hPD 1; hPD-1; hSLE1; PD 1; PD1; PDCD 1; PDCD1; Programmed cell death 1; Protein PD 1; Protein PD-1; SLEB2; Systemic lupus erythematosus susceptibility 2; PDCD1_HUMAN; Recombinant human PD-1 protein, C-His (HEK293) |
| 克 隆 号 | |
| 理论分子量 | kDa |
| 检测分子量 | Due to glycosylation, the protein migrates to 35-55 kDa based on Tris-Bis PAGE result. |
| 性 状 | Lyophilized |
| 浓 度 | ＞0.5 mg/ml |
| 物 种 | Human |
| 序 列 | 25-167/288 |
| 纯 度 | >95% as determined by Tris-Bis PAGE; >95% as determined by SEC-HPLC |
| 纯化方法 | AC |
| 内毒素 | <1.0 EU/μg as determined by LAL |
| 表达系统 | HEK293 cell |
| 活性 | Not tested |
| 标签 | C-His |
| 缓 冲 液 | Lyophilized from 0.22um filtered solution in PBS (pH7.4) with 5mM DTT. Normally 5% trehalose is added as protectant before Lyophilization. |
| 保存条件 | Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 | |
| 产品图片 |
Immobilized Human PD-1 ,His Tag at 0.5ug/ml(100ul/Well). Dose response curve for Anti-PD-1 Ab. with the EC50 of 45.0/41.6/45.7ng/ml determined by ELISA.
Immobilized Human PD-1,His Tag at 0.5μg/ml (100ul/Well). Dose response curve for Anti-PD-1 Ab. with the EC50 of 45.0ng/ml determined by ELISA.
The purity of PD-1 is greater than 95% as determined by SEC-HPLC.
Recombinant PD-1 on Tris-Bis PAGE under reduced . The purity is greater than 95%.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
