[IF=9.429] Zhang Yufan. et al. Scalable and high-throughput production of an injectable platelet-rich plasma (PRP)/cell-laden microcarrier/hydrogel composite system for hair follicle tissue engineering. J NANOBIOTECHNOL. 2022 Dec;20(1):1-22  WB ;  Mouse.  

PubMed:36329527

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1: SH-SY5Y, 2: Mouse cerebellum, 3: Rat cerebellum
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 95 kDa
Observed MW: 140-180 kDa

Western blot analysis of NCAM/CD56 on SH-SY5Y cell lysates with Rabbit anti-NCAM/CD56 antibody (bsm-52824R) at 1/500 dilution.

Lysates/proteins at 10 µg/Lane.

Predicted band size: 95 kDa
Observed band size: 120/140 kDa

Exposure time: 2 minutes;

6% SDS-PAGE gel.

Western blot analysis of NCAM/CD56 on human brain tissue lysates with Rabbit anti-NCAM/CD56 antibody (bsm-52824R) at 1/2,000 dilution.

Lysates/proteins at 20 µg/Lane.

Predicted band size: 95 kDa
Observed band size: 140/180 kDa

Exposure time: 30 seconds;

6% SDS-PAGE gel.

Paraformaldehyde-fixed, paraffin embedded (human Prostate); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (NCAM) Monoclonal Antibody, Unconjugated (bsm-52824R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (NCAM) Monoclonal Antibody, Unconjugated (bsm-52824R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (NCAM) Monoclonal Antibody, Unconjugated (bsm-52824R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (NCAM) Monoclonal Antibody, Unconjugated (bsm-52824R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (NCAM) Monoclonal Antibody, Unconjugated (bsm-52824R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (NCAM ) monoclonal Antibody, Unconjugated (bsm-52824R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.