| 产品编号 | bsm-60005R |
| 英文名称 | Mesothelin |
| 中文名称 | 间皮素重组兔单抗 |
| 别 名 | MSLN; MES; CAK 1; CAK1; CAK1 antigen; Megakaryocyte potentiating factor; Mesothelin isoform 1 precursor; MPF; Pre pro megakaryocyte potentiating factor; SMR; SMRP; Soluble MPF mesothelin related protein; MSLN_HUMAN. |
| 研究领域 | 肿瘤 免疫学 肿瘤细胞生物标志物 |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 交叉反应 | Human |
| 产品应用 | WB=1:500-2000, IHC-P=1:1000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 64kDa |
| 细胞定位 | 细胞膜 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Mesothelin |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Mesothelin(MSLN)may play a role in cell adhesion and cell shape dynamics; human homolog highly expressed in renal cell carcinoma. malignant tumors, such as a skin lump, ovary cancer and the pancreas cancer...etc..Mainly used for the research of the malignant tumor. Function: Membrane-anchored forms may play a role in cellular adhesion. Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro. Subunit: Interacts with MUC16. Subcellular Location: Cell membrane; Lipid-anchor, GPI-anchor. Golgi apparatus. Megakaryocyte-potentiating factor: Secreted. Isoform 3: Secreted. Tissue Specificity: Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level). Post-translational modifications: Both MPF and the cleaved form of mesothelin are N-glycosylated. Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin. Similarity: Belongs to the mesothelin family. SWISS: Q13421 Gene ID: 10232 Database links: Entrez Gene: 10232 Human Omim: 601051 Human SwissProt: Q13421 Human Unigene: 408488 Human |
| 产品图片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Lysate: HeLa Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 69 kDa Observed MW: 40, 69 kDa 
Tissue:Human endometrium
Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for bsm-60005R 
Paraformaldehyde-fixed, paraffin embedded (Human skin tumor); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Mesothelin) Monoclonal Antibody, Unconjugated (bsm-60005R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Mesothelin) Monoclonal Antibody, Unconjugated (bsm-60005R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue:Human cervical carcinoma
Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for bsm-60005R |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
