| 产品编号 | bsm-60016R |
| 英文名称 | MUC2 |
| 中文名称 | 粘蛋白-2/上皮膜抗原2重组兔单抗 |
| 别 名 | MLP; SMUC; Intestinal mucin 2; MUC 2; Mucin 2; Mucin 2 intestinal; Mucin 2 intestinal/tracheal; Mucin 2 oligomeric mucus/gel forming; Mucin 2 precursor; Mucin 2 tracheal; Mucin like protein; Mucin2; Intestinal mucin-2; MUC-2; Muc2; MUC2_HUMAN; Mucin-2; Mucin2. |
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Specific References (2) | bsm-60016R has been referenced in 2 publications.
[IF=5.988] Yang Liu. et al. Platycodon grandiflorus polysaccharides deeply participate in the anti-chronic bronchitis effects of platycodon grandiflorus decoction, a representative of "the lung and intestine are related". FRONT PHARMACOL. 2022 Sep 7;13:927384 IHC ; Rat.
[IF=3.829] Wang Ruibiao. et al. Effects of exosomes derived from Trichinella spiralis infective larvae on intestinal epithelial barrier function. VET RES. 2022 Dec;53(1):1-11 WB ; Pig.
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| 研究领域 | 肿瘤 细胞生物 免疫学 细胞粘附分子 糖蛋白 肿瘤细胞生物标志物 细胞外基质 |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | 7F9 |
| 交叉反应 | Rat,Human (predicted: Mouse) |
| 产品应用 | WB=1:500-1000, IHC-P=1:200-1:500, Flow-Cyt=1:100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 570kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MUC2 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins produced by many epithelial tissues. The protein encoded by this gene is secreted and forms an insoluble mucous barrier that protects the gut lumen. The protein polymerizes into a gel of which 80% is composed of oligosaccharide side chains by weight. The protein features a central domain containing tandem repeats rich in threonine and proline that varies between 50 and 115 copies in different individuals. Alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jul 2008] Function: Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer. Subunit: Homotrimer; disulfide-linked. Dimerizes in the endoplasmic reticulum via its C-terminal region and polymerizes via its N-terminal region by disulfide-linked trimerization. Interacts with FCGBP. Interacts with AGR2; disulfide-linked. Subcellular Location: Secreted. Note=In the intestine, secreted into the inner and outer mucus layers. Tissue Specificity: Colon, small intestine, colonic tumors, bronchus, cervix and gall bladder. Post-translational modifications: O-glycosylated. May undergo proteolytic cleavage in the outer mucus layer of the colon, contributing to the expanded volume and loose nature of this layer which allows for bacterial colonization in contrast to the inner mucus layer which is dense and devoid of bacteria. At low pH of 6 and under, undergoes autocatalytic cleavage in vitro in the N-terminal region of the fourth VWD domain. It is likely that this also occurs in vivo and is triggered by the low pH of the late secretory pathway. Similarity: Contains 1 CTCK (C-terminal cystine knot-like) domain. Contains 1 TIL (trypsin inhibitory-like) domain. Contains 2 VWFC domains. SWISS: Q02817 Gene ID: 4583 Database links: Entrez Gene: 4583 Human Entrez Gene: 17831 Mouse SwissProt: Q02817 Human SwissProt: Q80Z19 Mouse 细胞粘附蛋白（Call Adhesion Protein） 上皮膜抗原是粘性糖蛋白家族之一,是一种膜内在糖蛋白。Muc-2表达仅限于内膜腺上皮和腔上皮,很多上皮细胞及其来源的肿瘤表达该蛋白.其合成和分泌是腺上皮组织的特征之一。MUC-2也是一种主要的肠道粘蛋白，在正常肠道和结肠癌、胃癌等肿瘤中有广泛分布。该抗体主要用于胃肠道肿瘤的研究。 |
| 产品图片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: Rat colon Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 540 kDa Observed MW: 140-170, ＞250 kDa 
Paraformaldehyde-fixed, paraffin embedded (Human duodenum ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (MUC2) Monoclonal Antibody, Unconjugated (bsm-60016R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (MUC2) Monoclonal Antibody, Unconjugated (bsm-60016R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (MUC2) Monoclonal Antibody, Unconjugated (bsm-60016R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Cell line: HeLa
Fixative: 4% Paraformaldehyde
Permeabilization: 90% methanol
Primary ab dilution: 1:100
Secondary ab: Goat anti Rabbit IgG
Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line).
Isotype control: Rabbit monoclonal IgG (Blue line).
Comment: Line red is the positive signal for bsm-60016R
Cell line: NIH/3T3
Fixative: 4% Paraformaldehyde
Permeabilization: 90% methanol
Primary ab dilution: 1:100
Secondary ab: Goat anti Rabbit IgG
Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line).
Isotype control: Rabbit monoclonal IgG (Blue line).
Comment: Line red is the positive signal for bsm-60016R
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
