| 产品编号 | bsm-60528M |
| 英文名称 | WNT10B Recombinant Mouse mAb |
| 中文名称 | 信号通路WNT10B重组鼠单抗 |
| 别 名 | SHFM6; STHAG8; WNT-12; Wnt12; WN10B_HUMAN; WNT10B; Protein Wnt-12; WN10B_MOUSE; Wnt-10b; Wnt10; |
| 研究领域 | 肿瘤 免疫学 信号转导 转录调节因子 |
| 抗体来源 | Mouse |
| 克隆类型 | Recombinant |
| 克 隆 号 | 10G9 |
| 交叉反应 | Human,Mouse |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 40 kDa |
| 检测分子量 | 43 |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human WNT10B |
| 亚 型 | IgG1, Kappa |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It may be involved in breast cancer, and its protein signaling is likely a molecular switch that governs adipogenesis. This protein is 96% identical to the mouse Wnt10b protein at the amino acid level. This gene is clustered with another family member, WNT1, in the chromosome 12q13 region. [provided by RefSeq, Jul 2008] SWISS: O00744 Gene ID: 7480 |
| 产品图片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:10000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: 1: HeLa, 2: SH-SY5Y, 3: C2C12
Protein loading quantity: 20 μg
Exposure time: 3 s
Predicted MW: 43 kDa
Observed MW: 43 kDa
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
