| 产品编号 | bsm-60165M |
| 英文名称 | Ubiquityl-Histone H2B (Lys120) Mouse mAb |
| 中文名称 | 泛素化组蛋白H2B (Lys120)单克隆抗体 |
| 别 名 | H2BC3 | Histone H2B (ub-K120); ub-Histone H2B; ubiquityl-Histone H2B; H2BK120Ub; ub-H2B; H2B.1; H2B/f; H2BFF; HIST1H2BB; H2B1B_HUMAN; H2BC3; H2B-clustered histone 3; Histone H2B.1; Histone H2B.f (H2B/f); |
| 研究领域 | 细胞生物 神经生物学 信号转导 生长因子和激素 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse (predicted: Rat) |
| 产品应用 | WB=1:1000-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 14 kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Ubiquityl-Histone H2B (Lys120) |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008]. SWISS: P33778 Gene ID: 3018 |
| 产品图片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 4°C overnight
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: HeLa, N2a, BRL, Mouse liver， Mouse kidney
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 23 kDa
Observed MW: 23 kDa
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
