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Mouse Anti-Histone H2B (Acetyl K5)  antibody (bsm-60045M)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
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说明书: 50ul  100ul  
50ul/1580.00元
100ul/2500.00元
大包装/询价

产品编号 bsm-60045M
英文名称 Histone H2B (Acetyl K5)
中文名称 乙酰化化组蛋白H2B (Lys5)鼠单克隆抗体
别    名 H2BK5ac; Histone H2B (Acetyl Lys5); Acetyl Histone H2B (K5); acetyl-Histone H2B (Lys5); Histone H2B/HIST1H2BD; H2B.1; H2B.1 B; H2B.b; H2B.c; H2B.d; H2B.e; H2B.f; H2B.j; H2B.q; H2BFB; H2BFC; H2BFD; H2BFE; H2BFF; H2BFJ; H2BFO; H2BFQ; H2BFS; HIRIP2; HIST1H2BB; HIST1H2BD; HIST1H2BH; HIST1H2BL; HIST1H2BM; HIST1H2BN; HIST2H2BE; Histone H2B; Histone H2B type 1 B; Histone H2B type 1 D; Histone H2B type 1 H; Histone H2B type 1 L; Histone H2B type 1 M; Histone H2B type 1 N; Histone H2B type 2 E; Histone protein; H2B GL105; H2B histone family member O; H2B histone family member S; histone H2B type 1-B; H2B1B_HUMAN.  
产品类型 乙酰化抗体 
研究领域 表观遗传学  
抗体来源 Mouse
克隆类型 Monoclonal
克 隆 号 H2F3
交叉反应 Human (predicted: Mouse,Rat)
产品应用 WB=1:500-2000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 14kDa
细胞定位 细胞核 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Histone H2B (Acetyl K5) 
亚    型 IgG
纯化方法 affinity purified by Protein G
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008].

SWISS:
P33778

Gene ID:
3018

产品图片
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: (-) HeLa, (+) HeLa+Sodium butyrate (30mM, 4hr)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 14 kDa
Observed MW: 14 kDa
IP of HeLa+Sodium butyrate (30mM, 4hr) cells extracts
IP ab incubation condition: bsm-60045M, 4°C overnight, 1:3, 1:12 dilution
WB primary ab incubation condition: bsm-60045M, room temperature 2h, 1:2000 dilution
Secondary ab: Anti-Mouse IgG for IP (HRP)
Blocking buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM/TBST
Lane 1: 5% Input
Lane 2: IP with bsm-60045M (1:3)
Lane 3: IP with bsm-60045M (1:12)
Observed MW: 14 kDa
Exposure time: 60 s
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