| 产品编号 | bs-41337R |
| 英文名称 | AMPK alpha-1 Rabbit pAb |
| 中文名称 | 腺苷单磷酸活化蛋白激酶α1抗体 |
| 别 名 | AMPKalpha1; C130083N04Rik; AAPK1_MOUSE; Prkaa1; AMPK subunit alpha-1; Acetyl-CoA carboxylase kinase (ACACA kinase); Hydroxymethylglutaryl-CoA reductase kinase (HMGCR kinase); Tau-protein kinase PRKAA1; 2.7.11.1; AAPK1_RAT; Ampk1; AMPK; AMPK alpha 1; AMPKa |
| 研究领域 | 细胞生物 神经生物学 激酶和磷酸酶 Alzheimer's |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human (predicted: Mouse,Rat) |
| 产品应用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 64 kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant human AMPK alpha-1 protein : 1-207/559 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008] SWISS: Q13131/P54646 Gene ID: 5562 Database links:
Entrez Gene: 5562 Human
Entrez Gene: 105787 Mouse Omim: 602739 Human SwissProt: Q13131 Human SwissProt: Q5EG47 Mouse Unigene: 43322 Human Unigene: 207004 Mouse Unigene: 87789 Rat |
| 产品图片 |
Sample:
Lane 1: Human HUVEC cell lysates
Lane 2: Human SH-SY5Y cell lysates
Primary: Anti-AMPK alpha-1 (bs-41337R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDa
Measured by its binding ability in a indirect ELISA. Immobilized Human AMPK alpha-1 protein, His Tag (Cat. bs-41337P) at 2 μg/mL (100 μL/well) can bind Rabbit Anti-Human AMPK alpha-1 Antibody, the EC50 is 133.7 ng/mL.
Measured by its binding ability in a indirect ELISA. Immobilized Human AMPK alpha-1 protein, His Tag (Cat. bs-41337P) at 2 μg/mL (100 μL/well) can bind Rabbit Anti-Human AMPK alpha-1 Antibody, the EC50 is 13.01 ng/mL.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
