| 产品编号 | bs-42007R |
| 英文名称 | NDV HN |
| 中文名称 | 鸡新城疫血凝素抗体 |
| 别 名 | hemagglutinin-neuraminidase protein; newcastle disease virus(NDV); HN protein; hemagglutinin-neuraminidase protein [Newcastle disease virus]; HN_NDVB; Newcastle disease virus HN protein; Newcastle disease virus hemagglutinin neuraminidase protein. |
| 研究领域 | 免疫学 细菌及病毒 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | NDV |
| 产品应用 | WB=1:500-2000, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 63kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant NDV HN protein: 49-577/577 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The entry of Newcastle disease virus (NDV), a prototype paramyxovirus, is directed by two virion glycoproteins, the hemagglutinin-neuraminidase (HN) protein and the fusion (F) protein . HN protein, the virus attachment protein, binds to sialic acid-containing receptors, and F protein mediates membrane fusion. In contrast to many viral fusion proteins, paramyxovirus F proteins do not require the acid pH of endosomes to activate fusion activity. As a consequence, infected cells expressing both attachment proteins and F proteins can fuse with adjacent cells to form multinuclear cells, or syncytia, a process that is assumed to mimic virus-cell fusion . SWISS: P32884 Gene ID: N/A Database links: SwissProt: P32884 NDV |
| 产品图片 |
Sample:
Lane 1: Recombinant NDV HN protein, His Primary: Anti-NDV HN (bs-42007R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 63 kDa Observed band size: 61 kDa 
Measured by its binding ability in a indirect ELISA. Immobilized Human NDV HN protein, His Tag (Cat. bs-42007P) at 2 μg/mL (100 μL/well) can bind Rabbit Anti-Human NDV HN Antibody, the EC50 is 51.59 ng/mL.
Measured by its binding ability in a indirect ELISA. Immobilized Human NDV HN protein, His Tag (Cat. bs-42007P) at 2 μg/mL (100 μL/well) can bind Rabbit Anti-Human NDV HN Antibody, the EC50 is 54.90 ng/mL.
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
