| 产品编号 | bs-33011R |
| 英文名称 | RFP |
| 中文名称 | 红色荧光蛋白单克隆抗体 |
| 别 名 | Red fluorescent protein; Red fluorescent protein eqFP611; RFPTag; RFP-Tag; RFP Tag; RFP_ENTQU. |
| 产品类型 | 标签抗体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 产品应用 | WB=1:500-2000, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant Red fluorescent protein |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.Red Fluorescent Protein (RFP) is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. RFP can be excited by the 488 nm or 532 nm laser line and is optimally detected at 588 nm. SWISS: Q8ISF8 Gene ID: N/A |
| 产品图片 |
Sample:
Lane 1: Recombinant RFP protein, His Lane 2: Tags Positive Control Whole Cell Lysate, C-His Primary: Anti-RFP (bs-33011R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: kDa Observed band size: 30,44 kDa 
Measured by its binding ability in a indirect ELISA. Immobilized Human RFP protein, His Tag (Cat. bs-33011P) at 2 μg/mL (100 μL/well) can bind Rabbit Anti-Human RFP Antibody, the EC50 is 147.7 ng/mL.
Measured by its binding ability in a indirect ELISA. Immobilized Human RFP protein, His Tag (Cat. bs-33011P) at 2 μg/mL (100 μL/well) can bind Rabbit Anti-Human RFP Antibody, the EC50 is 161.8 ng/mL.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
