| 产品编号 | bs-4127R-AF750 |
| 英文名称 | PAP2B, Alexa Fluor 750 conjugated |
| 中文名称 | AF750标记的磷脂酸磷酸酶2b抗体 |
| 别 名 | Dri42; Lipid phosphate phosphohydrolase 3; LPP3; PAP 2b; PAP2 beta; PAP2b; Vascular endothelial growth factor and type I collagen inducible protein; VCIP. |
| 研究领域 | 肿瘤 免疫学 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 34kDa |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PAP2B: 241-311/311 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Phosphatidic acid phosphatase type 2b (PPAP2b) is a member of the phosphatidic acid phosphatase (PAP) family that function in de novo synthesis of glycerolipids as well as in receptor activated signal transduction mediated by phospholipase D. PAP2b catalyzes the conversion of phosphatidic acid to diacylglycerol and hydrolyzes lysophosphatidic acid, ceramide-1-phosphate and sphingosine-1-phosphate. It is a homodimer glycoprotein localized at the cell plasma membrane. Expression promotes adhesion, spreading, and tyrosine phosphorylation of Fak, Shc, Cas and paxillin in endothelial cells. PAP2b induced cell-cell interactions regulate specific intracellular signaling pathways that involve p120-catenin, Fak, Akt, and GSK3? protein kinases. SWISS: O14495 Gene ID: 8613 Database links: Entrez Gene: 8613 Human Entrez Gene: 67916 Mouse Omim: 607125 Human SwissProt: O14495 Human SwissProt: Q99JY8 Mouse Unigene: 405156 Human Unigene: 619002 Human Unigene: 348326 Mouse Unigene: 12038 Rat |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
