| 产品编号 | bsm-60613R |
| 英文名称 | CD25 Recombinant Rabbit mAb |
| 中文名称 | 白介素2受体a链/ICD25重组兔单抗 |
| 别 名 | CD25; Il2r; Ly-43; IL2RA_MOUSE; Il2ra; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA; p55; IDDM10; IMD41; TCGFR; |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | H12C8 |
| 交叉反应 | Human |
| 产品应用 | WB=1:500-2000,Flow-Cyt=1ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 | 白细胞介素 2 (IL2) 受体α (IL2RA) 和β (IL2RB) 链与共同的γ 链(IL2RG) 一起构成高亲和力IL2 受体。同源二聚体 α 链 (IL2RA) 产生低亲和力受体，而同源二聚体 β (IL2RB) 链产生中等亲和力受体。通常，一种完整的膜蛋白、可溶性 IL2RA 已被分离并确定是由细胞外蛋白水解产生的。交替剪接的 IL2RA mRNA 已被分离出来，但每个的意义目前尚不清楚。该基因的突变与白细胞介素 2 受体 α 缺陷有关。2019 年严重冠状病毒病 (COVID-19)（由新型严重急性呼吸系统综合症冠状病毒 2（SARS-CoV-2）引起的疾病）患者血浆中的 IL2R 水平显着升高。同样，发现不同类型癌症患者的血清 IL-2R 水平升高。某些 IL2RA 和 IL2RB 基因多态性与肺癌风险相关。 |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with CD25 monoclonal antibody, unconjugated (bsm-60613R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The Jurkat (treated with 20ng/ml PMA for 24 hours) (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.) , followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-CD25 antibody (bsm-60613R): 1 μg/10^6 cells; Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
