| 产品编号 | bsm-60442R |
| 英文名称 | PTGER2 Recombinant Rabbit mAb |
| 中文名称 | |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | F3C1 |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 亚 型 | IgG/Kappa |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 | 前列腺素E2（PGE2）受体，该受体的活性由刺激腺苷酸环化酶的G（s）蛋白介导。随后细胞内cAMP的升高是该受体对平滑肌产生舒张作用的原因。 |
| 产品图片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 4°C overnight
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: HeLa, JAR, F9, Rat liver
Protein loading quantity: 20 μg
Exposure time: 10 s
Predicted MW: 52 kDa
Observed MW: 56 kDa
Tissue: Mouse liver
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60442R
Tissue: Human placenta
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60442R
Tissue: Rat lung
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60442R
Cell line: HeLa
Fixative:100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for bsm-60442R
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
