| 产品编号 | bs-60626R |
| 英文名称 | PAK3 Rabbit pAb |
| 中文名称 | p21激活激酶3抗体 |
| 别 名 | ARA; MRX30; MRX47; OPHN3; PAK-3; PAK3beta; XLID30; bPAK; beta-PAK; Pak65alpha; Pak65beta; Stk4; PAK3_HUMAN; PAK3; Oligophrenin-3; p21-activated kinase 3 (PAK-3); 2.7.11.1; PAK3_MOUSE; CDC42/RAC effector kinase PAK-B; Pakb; PAK3_RAT; p65-PAK; |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 | 丝氨酸/苏氨酸蛋白激酶，在多种不同的信号通路中发挥作用，包括细胞骨架调节、细胞迁移或细胞周期调节。在树突棘形态发生以及突触形成和可塑性中起作用。充当小GTP酶CDC42和RAC1的下游效应器。活性CDC42和RAC1结合的激活导致构象改变和随后几个丝氨酸和/或苏氨酸残基的自磷酸化。磷酸化MAPK4和MAPK6并激活下游靶MAPKAPK5，后者是F-肌动蛋白聚合和细胞迁移的调节因子。此外，磷酸化TNNI3/肌钙蛋白I以调节薄肌丝的钙敏感性和松弛动力学。 |
| 产品图片 |
Tissue: Human cerebellum
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bs-60626R
Tissue: Human cerebrum
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bs-60626R
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
