产品编号 | bs-60626R |
英文名称 | PAK3 |
中文名称 | |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | (predicted: Human) |
产品应用 | IHC-P=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
细胞定位 | 细胞膜 |
性 状 | Liquid |
浓 度 | 1mg/ml |
亚 型 | IgG |
纯化方法 | Affinity purified by Protein A |
缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 | 丝氨酸/苏氨酸蛋白激酶,在多种不同的信号通路中发挥作用,包括细胞骨架调节、细胞迁移或细胞周期调节。在树突棘形态发生以及突触形成和可塑性中起作用。充当小GTP酶CDC42和RAC1的下游效应器。活性CDC42和RAC1结合的激活导致构象改变和随后几个丝氨酸和/或苏氨酸残基的自磷酸化。磷酸化MAPK4和MAPK6并激活下游靶MAPKAPK5,后者是F-肌动蛋白聚合和细胞迁移的调节因子。此外,磷酸化TNNI3/肌钙蛋白I以调节薄肌丝的钙敏感性和松弛动力学。 |
产品图片 |
Tissue: Human cerebellum
Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bs-60626R
Tissue: Human cerebrum
Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bs-60626R |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |