| 产品编号 | bs-60204R |
| 英文名称 | Malonyl-Histone H3 (Lys122) Rabbit pAb |
| 中文名称 | 丙二酰化组蛋白H3K122抗体 |
| 别 名 | H3C1 | Histone H3 (mal-K122); mal-Histone H3; malonyl-Histone H3; H3K122Mal; mal-H3; H3/A; H3C10; H3C11; H3C12; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FA; HIST1H3A; H3/b; H3C1; H3FB; HIST1H3D; H3.1; H3/c; H3FC; HIST1H3C; H3/d; H3FD; HIST1H3E; H3.f; H3/f; H |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 | 赖氨酸丙二酰化是一种最近发现的可逆修饰，可调控蛋白质活性。Sirt5是一种赖氨酸丙二酸化修饰调节酶，可催化体内外赖氨酸脱丙二酰化。 整合蛋白质组学和生物化学分析技术发现，原核生物和真核生物中，不管是组蛋白还是非组蛋白都可发生赖氨酸丙二酰化。赖氨酸丙二酰化可能为研究胞内调节如各种生物体中的能量代谢提供新的典范。 |
| 产品图片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: (-) HeLa, (+) HeLa+Sodium malonate (50nM, 72hr)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 17 kDa
Observed MW: 17 kDa
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
