| 产品编号 | bsm-52537R |
| 英文名称 | GART Recombinant Rabbit mAb |
| 中文名称 | 甘氨酰胺核苷酸合成酶重组兔单抗 |
| 别 名 | 5'-phosphoribosylglycinamide transformylase; AIR synthase; AIRS; GAR transformylase; GARS; GARTF; Glycinamide ribonucleotide synthetase; MGC47764; PAIS; PGFT; phosphoribosyl-aminoimidazole synthetase; phosphoribosylglycinamide formyltransferase; phosphoribosylglycinamide formyltransferase phosphoribosylglycinamide synthetase phosphoribosylaminoimidazole synthetase; phosphoribosylglycinamide formyltransferase, EC 2.1.2.29; phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase; phosphoribosylglycinamide synthetase; PRGS; PUR2_HUMAN; Trifunctional purine biosynthetic protein adenosine 3. |
| 研究领域 | 肿瘤 细胞生物 信号转导 新陈代谢 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | 6A8 |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 107 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant Human GART protein: 570-750/1010 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Purines are critical for energy metabolism, cell signaling and cell reproduction and also function as precursors for coenzymes, energy transfer molecules, regulatory factors and proteins involved in RNA and DNA synthesis. GART (GAR transformylase), also referred to as AIRS, GARS, PAIS, PGFT, PRGS or GARTF, is 1,010 amino acids in length and is a key folate-dependent trifunctional enzyme with phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase and AICAR (phosphoribosylaminoimidazole synthetase) activity required for de novo purine biosynthesis. Cancer cells require considerable amounts of purines to sustain their accelerated growth and GART is, therefore, a target for cancer chemotherapy. GART is highly conserved in vertebrates. Two isoforms of GART are expressed due to alternative splicing events. SWISS: P22102 Gene ID: 2618 Database links: Entrez Gene: 2618 Human Entrez Gene: 14450 Mouse Omim: 138440 Human SwissProt: P22102 Human SwissProt: Q64737 Mouse Unigene: 473648 Human Unigene: 4505 Mouse |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with GART monoclonal antibody, unconjugated (bsm-52537R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GART Monoclonal Antibody, Unconjugated (bsm-52537R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GART Monoclonal Antibody, Unconjugated (bsm-52537R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GART Monoclonal Antibody, Unconjugated (bsm-52537R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
