产品编号 | bsm-52389R |
英文名称 | N Cadherin |
中文名称 | N-钙粘附分子重组兔单抗 |
别 名 | Cadherin 2; Cadherin 2 N cadherin neuronal; Cadherin 2 type 1; Cadherin 2 type 1 N cadherin neuronal; cadherin 2 type 1 N-cadherin neuronal; Cadherin2; Calcium dependent adhesion protein neuronal; CD325; CD325 antigen; CDH2; CDHN; CDw325; CDw325 antigen; N cadherin 1; NCAD; Neural Cadherin; Neural Cadherin. |
研究领域 | 肿瘤 细胞生物 免疫学 激酶和磷酸酶 细胞粘附分子 |
抗体来源 | Rabbit |
克隆类型 | Recombinant |
克 隆 号 | 5G1 |
交叉反应 | Human,Mouse,Rat |
产品应用 | WB=1:1000-5000, IHC-P=1:100-500, IHC-F=1:400-800
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 100kDa |
细胞定位 | 细胞膜 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human N Cadherin: 150-250/905 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product. SWISS: P19022 Gene ID: 1000 Database links: Entrez Gene: 1000 Human Entrez Gene: 12558 Mouse Omim: 114020 Human SwissProt: P19022 Human SwissProt: P15116 Mouse Unigene: 464829 Human Unigene: 606106 Human Unigene: 257437 Mouse Unigene: 23200 Rat |
产品图片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Lysate: HeLa, Mouse brain, Rat brain Protein loading quantity: 20 μg Exposure time: 10 s Predicted MW: 100 kDa Observed MW: 125 kDa
Sample:
Lane 1: Mouse Heart tissue lysates Lane 2: Mouse Testis tissue lysates Lane 3: Rat Cerebrum tissue lysates Lane 4: Human PANC-1 cell lysates Lane 5: Human A549 cell lysates Primary: Anti-N Cadherin (bsm-52389R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 100 kDa Observed band size: 140 kDa
Tissue: Human endometrium
Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for bsm-52389R
Tissue: Human liver
Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for bsm-52389R
Tissue: Rat liver
Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for bsm-52389R
Tissue: Mouse liver
Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for bsm-52389R |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |