| 产品编号 |
bs-41498P |
| 英文名称 |
Recombinant CRISPR-Cas9 protein, C-His
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| 中文名称 |
重组抗CRISPR-Cas9蛋白 |
| 别 名 |
Cas9 ; CRISPR-associated endonuclease Cas9/Csn1 ; CRISPR-Cas9/Csn1 ; csn1 ; SpyCas9. |
| 克 隆 号 |
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| 理论分子量 |
164 kDa |
| 检测分子量 |
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| 性 状 |
Lyophilized or Liquid |
| 浓 度 |
0.3mg/ml |
| 物 种 |
Streptococcus pyogenes |
| 序 列 |
2-1368/1368 |
| 纯 度 |
> 90% as determined by SDS-PAGE |
| 表达系统 |
E.coli |
| 活性 |
Not tested |
| 标签 |
C-His |
| 缓 冲 液 |
PBS (pH7.4). |
| 保存条件 |
Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles. |
| 注意事项 |
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.
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| 产品图片 |
The purity of the protein is greater than 90% as determined by reducing SDS-PAGE.
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