| 产品编号 | bsm-60696R |
| 英文名称 | ATG9A Recombinant Rabbit mAb |
| 中文名称 | 自噬相关蛋白9A重组兔单抗 |
| 别 名 | ATG9A_HUMAN; Autophagy-related protein 9A; APG9-like 1; mATG9; APG9L1; MGD3208; |
| 研究领域 | 肿瘤 细胞生物 信号转导 肿瘤细胞生物标志物 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | R4D8 |
| 交叉反应 | Human |
| 产品应用 | WB=1:500-1000,IHC-P=1:100-500,IHC-F=1:50-100,IF=1:50-100,ICC/IF=1:50-100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 94 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ATG9A |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Acts upstream of or within autophagosome assembly. Located in endosome; phagophore assembly site; and trans-Golgi network. [provided by Alliance of Genome Resources, Apr 2022] Subcellular Location: Cytoplasmic vesicle > autophagosome membrane. Golgi apparatus > trans-Golgi network membrane. Late endosome membrane. SWISS: Q7Z3C6 Gene ID: 79065 Database links: Entrez Gene: 79065 Human SwissProt: Q7Z3C6 Human |
| 产品图片 |
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at
room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L
(HRP)
Lysate: 1: HepG2, 2: HeLa
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 94 kDa
Observed MW: 100 kDa
Tissue: Human thyroid cancer
Section type: Formalin-fixed & Paraffin
embedded section
Retrieval method: High temperature and high
pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at
room temperature
Secondary Ab: Anti-Rabbit and Mouse
Polymer HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal
for bsm-60696R
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
