| 产品编号 | bsm-60753M |
| 英文名称 | Citrate synthase |
| 中文名称 | 柠檬酸合成酶CISY单克隆抗体 |
| 别 名 | CISY_HUMAN; Citrate synthase, mitochondrial; Cs; EC 2.3.3.1; Citrate (Si)-synthase. 柠檬酸合酶CS |
| 研究领域 | 肿瘤 细胞生物 信号转导 新陈代谢 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | C9H4 |
| 交叉反应 | Rat,Mouse,Human |
| 产品应用 | WB=1:500-1000, IHC-P=1:100-500, ICC=1:50, IF=1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 52kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human citrate synthase |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | PBS, Glycerol, BSA |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is a Krebs tricarboxylic acid cycle enzyme that catalyzes the synthesis of citrate from oxaloacetate and acetyl coenzyme A. The enzyme is found in nearly all cells capable of oxidative metablism. This protein is nuclear encoded and transported into the mitochondrial matrix, where the mature form is found. [provided by RefSeq, Jul 2008] SWISS: O75390 Gene ID: 1431 |
| 产品图片 |
Sample:
Lane 1: Mouse Heart tissue lysates Lane 2: Mouse NIH/3T3 cell lysates Lane 3: Mouse Muscle tissue lysates Lane 4: Mouse Cerebrum tissue lysates Lane 5: Mouse Kidney tissue lysates Lane 6: Rat Heart tissue lysates Lane 7: Rat Muscle tissue lysates Lane 8: Rat Cerebrum tissue lysates Lane 9: Rat Kidney tissue lysates Lane 10: Human HeLa cell lysates Lane 11: Human 293T cell lysates Lane 12: Human HL-60 cell lysates Lane 13: Human HUVEC cell lysates Lane 14: Human HepG2 cell lysates Primary: Anti-Citrate synthase (bsm-60753M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti- Mouse IgG at 1/20000 dilution Predicted band size: 52 kDa Observed band size: 45 kDa |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
