| 产品编号 | bsm-60751R |
| 英文名称 | Rabbit Anti-Heme Oxygenase 1 antibody |
| 中文名称 | 血红素氧合酶 1抗体 |
| 别 名 | Heme oxygenase (decycling) 1; Heme oxygenase 1; Hemox; HMOX 1; Hmox; HMOX1; HO 1; HO-1; HO; HO1; Hsp32; bK286B10; D8Wsu38e; HMOX1_HUMAN. |
| 研究领域 | 肿瘤 心血管 免疫学 神经生物学 信号转导 表观遗传学 Alzheimer's |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | WB=1:2000-20000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1:50-100,ICC/IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 32kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Heme Oxygenase 1 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | PBS, Glycerol, BSA. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The hemeoxygenase-1 calls that the hemoglobin oxidizes to synthesize the enzyme again-1( hemeoxygenase-1, HO-1) is the catalyst enzyme that a kind of hemoglobin declines the solution, under the NADPH and the cell dye P-450 revivification enzymes and the member oxygen functions, the catalyst HO-1 hemoglobin declines the solution as the courage green vegetable, CO and irons, the former revivification has the very strong anti- to oxidize the ability after become the red vegetable of courage , the latter is a kind of important letter to make the member. SWISS: P09601 Gene ID: 3162 |
| 产品图片 |
Sample:
Lane 1: Mouse Liver tissue lysates
Lane 2: Mouse Placenta tissue lysates
Lane 3: Mouse NIH/3T3 cell lysates
Lane 4: Rat Spleen tissue lysates
Lane 5: Rat Liver tissue lysates
Lane 6: Human HeLa cell lysates
Lane 7: Human 293T cell lysates
Lane 8: Human HepG2 cell lysates
Primary: Anti-Heme Oxygenase 1 (bsm-60751R) at 1/10000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 32 kDa
Observed band size: 30 kDa
Paraformaldehyde-fixed, paraffin embedded (mouse thymus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Heme Oxygenase 1) Monoclonal Antibody, Unconjugated (bsm-60751R) at 1:1000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Heme Oxygenase 1) Monoclonal Antibody, Unconjugated (bsm-60751R) at 1:1000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Heme Oxygenase 1) Monoclonal Antibody, Unconjugated (bsm-60751R) at 1:1000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Heme Oxygenase 1) Monoclonal Antibody, Unconjugated (bsm-60751R) at 1:1000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat thymus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Heme Oxygenase 1) Monoclonal Antibody, Unconjugated (bsm-60751R) at 1:1000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Heme Oxygenase 1) Monoclonal Antibody, Unconjugated (bsm-60751R) at 1:1000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
