Sealing solution and concentration: 5% NFDM/TBST First antibody dilution: 1:1000 Incubation conditions for primary antibody: 2 hours at room temperature Cracking solution: 1: HeLa, 2: NIH-3T3, 3: BRL, 4: Mouse liver, 5: Mouse kidney Secondary antibody: Goat Anti Rabbit IgG H&L (HRP) Protein loading amount: 20 μ G Exposure time: 60 seconds Theoretical molecular weight: Multiple kDa Actual molecular weight: Multiple kDa

Sealing solution and concentration: 5% NFDM/TBST Cracking liquid: HeLa First antibody dilution: 1:1000 Incubation conditions for primary antibody: 2 hours at room temperature Secondary antibody: Goat Anti Rabbit IgG H&L (HRP) Processing method: 1: PTM-105RM pre adorbed with 3 μ M synthetic Kac peptides; 2: PTM-105RM pre adorbed with 3 μ M unmodified peptides Protein loading amount: 20 μ G Exposure time: 30 seconds Theoretical molecular weight: Multiple kDa Actual molecular weight: Multiple kDa

Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Acetyl Lysine Monoclonal Antibody, Unconjugated (bsm-60865R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Acetyl Lysine Monoclonal Antibody, Unconjugated (bsm-60865R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.