| 产品编号 | bs-42253R |
| 英文名称 | Endothelin 1 Rabbit pAb |
| 中文名称 | 内皮素-1抗体 |
| 别 名 | EDN1_HUMAN; Big endothelin 1; EDN 1; EDN1; EDN1 protein; Endothelin 1; Endothelin-1; ET 1; ET1; OTTHUMP00000039245; PPET1; Preproendothelin 1; Preproendothelin1. |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 22 kDa |
| 检测分子量 | |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant mouse Endothelin-1 protein: 26-202/202 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Endothelins (ET) show potent constrictor activity in vascular and non-vascular smooth muscle. This family of 21-amino acid peptides exists in at least three isoforms - ET-1, ET-2, and ET-3, and is produced in endothelial and epithelial cells. ET's can mediate biological effects in cells and tissues, and have been shown to bind to an ET receptor in the lung, kidney, heart, and liver. Endothelin 1 is expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells. Molecular weight: 2491.9 Molecular formula: C109H159N25O32S5 CAS: 117399-94-7 MDL: MFCD00133305 Reconstitution: 1mM (1mg/0.4013mL) Function: Endothelins are endothelium-derived vasoconstrictor peptides. Subcellular Location: Secreted. Tissue Specificity: Expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells. Similarity: Belongs to the endothelin/sarafotoxin family. SWISS: P22387 Gene ID: 13614 Database links: Entrez Gene: 1906 Human Entrez Gene: 13614 Mouse Omim: 131240 Human SwissProt: P05305 Human SwissProt: P22387 Mouse Unigene: 511899 Human Unigene: 713645 Human Unigene: 10918 Rat Endothelin-1 (human, porcine)多肽内皮素家族的三种异肽之一，是由活化的内皮细胞合成和分泌的，是一种具有强大血管收缩活性的肽，人和猪内皮素1序列同源，是有效的内源性血管收缩剂。Endothelin-1通过两种类型受体ETA和ETB发挥作用。Endothelin-1诱导缺氧诱导因子1α血管内皮生长因子的产生。除了血管收缩作用外，ET-1还会导致血管细胞纤维化，刺激活性氧的产生。研究显示，ET-1诱导促炎机制，增加超氧阴离子的产生和细胞因子的分泌。它还可由心肌细胞释放。它是活化或功能异常的内皮细胞的一种标志物。 |
| 产品图片 |
Sample:
Lane 1: Mouse Placenta tissue lysates
Lane 2: Mouse Lung tissue lysates
Primary: Anti-Endothelin 1 (bs-42253R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 23 kDa
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
