| 产品编号 | bs-42286P |
| 英文名称 | Recombinant human NFKB p65 protein, N-GST |
| 中文名称 | 重组人细胞核因子/k基因结合核因子蛋白 |
| 别 名 | kB P65; NF-kB p65; NFKBp65; NF-κBp65; NF-kBp65; Avian reticuloendotheliosis viral (v rel) oncogene homolog A; MGC131774; NFKB 3; NFKB3; Nuclear Factor NF Kappa B p65 Subunit; Nuclear factor of kappa light polypeptide gene enhancer in B cells 3; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B Cells; p65; p65 NF kappaB; p65 NFkB; RELA; Transcription Factor p65; v rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B cells 3 (p65)); V Rel Avian Reticuloendotheliosis Viral Oncogene Homolog A; v rel reticuloendotheliosis viral oncogene homolog A (avian); v-rel reticuloendotheliosis viral oncogene homolog A; p65NFKB; TF65_HUMAN. |
| 理论分子量 | 62.5kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | >0.5mg/ml |
| 物 种 | Human |
| 序 列 | 1-306/551 |
| 纯 度 | >90% as determined by SDS-PAGE |
| 纯化方法 | AC |
| 内毒素 | Not analyzed |
| 表达系统 | E.coli |
| 标签 | N-GST |
| 缓 冲 液 | 50mM Tris-Hcl (pH=8.0) with 10mM GSH |
| 保存条件 | Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]. SWISS: Q04206 Gene ID: 5970 转录调节因子（Transcriptin Regulators） NF-κBp65是一种重要的转录因子，NF-kBp65可激活参与炎症、细胞增殖、细胞凋亡等基因的调节，影响着细胞的凋亡，同时影响着肿瘤细胞对细胞毒性药物及离子辐射的敏感性。ras基因诱导的致癌突变作用需NFkB的活化，提示NFkB在致癌发生方面可能起一定作用；另有文献报道，在乳腺癌、非小细胞性肺癌、甲状腺癌、T或B淋巴细胞白血病及病毒诱变导致的肿瘤等人类肿瘤中，NFkB活化或表达。 经研究认为：NFKBp65蛋白在静息状态下以结合态的方式存在于胞浆中，当NFKBp65蛋白被激活后解离进入细胞核。(NF-κB与其抑制蛋白IκB家族成员结合，以无活性的复合物形式存在于胞浆中，当细胞受到各种刺激后，NF- κB与IκB 解离，从而进入细胞核，与相应的靶序列结合，调节基因的表达) |
| 产品图片 |
The purity of the protein is greater than 85% as determined by reducing SDS-PAGE.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
