| 产品编号 | bs-42326R |
| 英文名称 | HIF1A Rabbit pAb |
| 中文名称 | 缺氧诱导因子1α /低氧诱导因子-1抗体 |
| 别 名 | HIF-1-alpha; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; MOP1; PASD8; bHLHe78; HIF1alpha; HIF1A_HUMAN; HIF1A; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78 (bHLHe78); Member of PAS protein 1; PAS domain-containing protein 8; HIF1A_MOUSE; |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human |
| 产品应用 | ICC/IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 92 kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg、ml |
| 免 疫 原 | Recombinant human HIF-1 Alpha protein: 575-826/826 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
缺氧诱导因子1α不仅对于机体在缺氧条件下维持正常的生理功能具有特别重要的意义,并在肿瘤的生长以及神经细胞凋亡等病理过程中起重要作用. HIF1 alpha能调节许多下游基因的表达水平. 哺乳动物细胞在低氧压力条件下出现HIF。HIF是一种转录因子，对细胞的缺氧起稳定作用。 |
| 产品图片 |
4% Paraformaldehyde-fixed HepG2 (treated with 500uM CoCl2 for 6 hours) (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (HIF-1 Alpha) polyclonal Antibody, unconjugated (bs-42326R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
4% Paraformaldehyde-fixed Hela (treated with 500uM CoCl2 for 6 hours) (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (HIF-1 Alpha) polyclonal Antibody, unconjugated (bs-42326R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
