| 产品编号 | bs-42266R |
| 英文名称 | CD30 Rabbit pAb |
| 中文名称 | CD30抗体 |
| 别 名 | TNR8_HUMAN; TNFRSF8; CD30L receptor; Ki-1 antigen; Lymphocyte activation antigen CD30; CD30; D1S166E; |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 62 kDa |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant human CD30 protein: 20-379/595 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008] Function: Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B. Subunit: Interacts with TRAF1, TRAF2, TRAF3 and TRAF5. Subcellular Location: Isoform Long: Cell membrane; Single-pass type I membrane protein. Isoform Short: Cytoplasm. Tissue Specificity: Detected in thymus and in activated splenocytes. Post-translational modifications: Phosphorylated on serine and tyrosine residues. Similarity: Contains 6 TNFR-Cys repeats SWISS: P28908 Gene ID: 943 Database links: Entrez Gene: 943 Human Omim: 153243 Human SwissProt: P28908 Human Unigene: 1314 Human CD30是一种单链糖蛋白，标记活化T和B淋巴细胞，R-S细胞和部分淋巴滤泡间区免疫母细胞。CD30是霍奇金淋巴瘤的一个特异性标记，也是大细胞间变型淋巴瘤的诊断型标志。 |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with CD30 polyclonal antibody, unconjugated (bs-42266R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
