| 产品编号 | bs-47032P-Bio |
| 英文名称 | Recombinant human TNFRSF9 protein, C-His-Avi (HEK293), Biotin conjugated |
| 中文名称 | 生物素标记重组人TNFRSF9/CD137蛋白 |
| 别 名 | Tumor necrosis factor receptor superfamily member 9; TNFRSF9; 4-1BB; 4 1BB; 4 1BB ligand receptor; Homolog of mouse 4 1BB; Receptor protein 4 1BB; T cell antigen 4 1BB homolog; T cell antigen ILA; CDw137; CD 137; CD137 antigen; Induced by lymphocyte activation; ILA; Interleukin activated receptor homolog of mouse Ly63; Ly63; MGC2172; MGC114552; TNR9_HUMAN; Recombinant human TNFRSF9 protein, C-His-Avi (HEK293) / Biotin |
| 克 隆 号 | |
| 理论分子量 | 18 kDa |
| 检测分子量 | Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result. |
| 浓 度 | ＞0.5 mg/ml |
| 纯 度 | >95% as determined by Tris-Bis PAGE; >95% as determined by SEC-HPLC |
| 缓 冲 液 | Lyophilized from 0.22um filtered solution in PBS (pH7.4) with 5mM DTT. Normally 5% trehalose is added as protectant before Lyophilization. |
| 保存条件 | Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
CD137 exists on the cell surface as a monomer with a molecular mass of 30 kDa and as a dimer of 55 kDa. Human and mouse CD137 share 60% amino acid identity. CD137 (4-1BB), a member of the tumour necrosis factor receptor superfamily, is a type I transmembrane glycoprotein expressed on the cell surface of activated splenic T cells and thymocytes. The functions of CD137 in T lymphocytes include regulating activation, proliferation and apoptosis. CD137 and CD28 are costimulatory molecules of T cell activation. Costimulatory molecules are important in initiating anti-tumor immune responses. CD137 plays an important role in regulating T-cell-dependent immune responses. Expression of CD137 correlates negatively with lymphocyte proliferation and positively with the degree of activation-induced cell death caused by mitogen overstimulation. In monocytes, CD137 induces activation, promotes adherence and prolongs survival. CD137属于肿瘤坏死因子超家族成员，是可诱导的T细胞共刺激受体，表达于CD4 + 和CD8 + T细胞、活化的自然杀伤细胞（natural killercell，NK）和树突状细胞,CD137可抑制T细胞增殖，诱发细胞凋亡。 |
| 产品图片 |
Immobilized Human 4-1BB Ligand at 1μg/ml (100μl/Well). Dose response curve for Biotinylated Human 4-1BB with the EC50 of 0.38μg/ml determined by ELISA.
The purity of Biotinylated Human 4-1BB is greater than 95% as determined by SEC-HPLC.
Biotinylated Human 4-1BB on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
