产品编号 | bs-47071P-Bio |
英文名称 | Recombinant human/Cyn. monkey/rhesus macaque CD28 protein, N-His-Avi (HEK293), Biotin conjugated |
中文名称 | 生物素标记重组人/食蟹猴/恒河猴CD28蛋白 |
别 名 | CD 28; CD28 antigen; CD28 molecule; MGC138290; T cell antigen CD28; T cell specific surface glycoprotein; T cell specific surface glycoprotein CD28; Tp44; CD28_HUMAN; Recombinant human/Cyn. monkey/rhesus macaque CD28 protein, N-His-Avi (HEK293) / Biotin |
克 隆 号 | |
理论分子量 | 42.6 kDa |
检测分子量 | Due to glycosylation, the protein migrates to 38-50 kDa based on Tris-Bis PAGE result. |
浓 度 | >0.5 mg/ml |
纯 度 | >95% as determined by Tris-Bis PAGE; >95% as determined by SEC-HPLC |
缓 冲 液 | Lyophilized from 0.22um filtered solution in PBS (pH7.4) with 5mM DTT. Normally 5% trehalose is added as protectant before Lyophilization. |
保存条件 | Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
产品介绍 |
The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011] CD28是免疫球蛋白超家族成员之一 ,为T细胞特异性由二硫键连接的同源二聚体表面糖蛋白,主要分布在外周血淋巴细胞。对迟发型超敏反应以及Tc介导的细胞毒作用都有免疫调节作用。 |
产品图片 |
Immobilized Human / Cynomolgus / Rhesus macaque CD28,His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD28 Ab, hFc Tag with the EC50 of 17.3ng/ml determined by ELISA.
The purity of Human / Cynomolgus / Rhesus macaque CD28 Protein is greater than 95% as determined by SEC-HPLC.
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |