| 产品编号 | bs-47168P-Bio |
| 英文名称 | Recombinant human Notch 1 protein, C-His-Avi (HEK293), Biotin conjugated |
| 中文名称 | 生物素标记重组人Notch 1蛋白 |
| 别 名 | EC 2.1.2.11; EC 3.4.21.68; Neurogenic locus notch homolog protein 1; Notch 1 extracellular truncation; Notch 1 intracellular domain; hN1; Lin-12; LIN12; MIS6; Motch A; mT14; Neurogenic locus notch homolog protein 1; Neurogenic locus notch protein homolog; NICD; Notch1 intracellular domain; NOTC1_HUMAN; Notch 1; NOTCH; Notch gene homolog 1 (Drosophila); Notch homolog 1 translocation associated (Drosophila); Notch homolog 1, translocation-associated (Drosophila); NOTCH, Drosophila, homolog of, 1; p300; TAN 1; TAN1; Translocation Associated Notch Homolog; Translocation Associated Notch Homolog; Translocation associated notch protein TAN 1; Translocation-associated notch protein TAN-1; Recombinant human Notch 1 protein, C-His-Avi (HEK293) / Biotin |
| 克 隆 号 | |
| 理论分子量 | 56.5 kDa |
| 检测分子量 | Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result. |
| 浓 度 | ＞0.5 mg/ml |
| 纯 度 | >95% as determined by Tris-Bis PAGE; >95% as determined by SEC-HPLC |
| 缓 冲 液 | Lyophilized from 0.22um filtered solution in PBS (pH7.4) with 5mM DTT. Normally 5% trehalose is added as protectant before Lyophilization. |
| 保存条件 | Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play multiple roles during development. [provided by RefSeq, Jul 2008]. Notch 1蛋白是一个进化上保守的跨膜受体家族，广泛分布和表达在多个物种之中。研究发现Notchl作为一条信号转导途径，不仅对正常组织、细胞的分化、发育起重要作用，而且和一些肿瘤的发生和生长相关，有学者发现Notchl在许多实体瘤中异常表达，如：如宫颈癌、子宫内膜癌、肾癌、肺癌、乳腺癌、神经母细胞瘤等。因此Notchl作为一种预防和治疗肿瘤的新途径越来越受到人们的重视。 |
| 产品图片 |
The purity of Biotinylated Human Notch 1 Protein is greater than 95% as determined by SEC-HPLC.
Biotinylated Recombinant Human Notch 1 Protein on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
