| 产品编号 | bs-47219P-Bio |
| 英文名称 | Recombinant human TNFRSF10D protein, C-His-Avi (HEK293), Biotin conjugated |
| 中文名称 | 生物素标记重组人TNFRSF10D蛋白 |
| 别 名 | DcR2; Decoy receptor 2; CD 264; CD264; CD-264; DcR 2; decoy with truncated death domain; ID; TNF receptor related receptor for TRAIL; TNF related apoptosis inducing ligand receptor 4; TNFRSF 10D; TRAIL R4; TRAIL receptor 4; TRAIL receptor with a truncated death domain; TRAILR4; TRIALR 4; TRUNDD; Tumor necrosis factor receptor superfamily member 10d decoy with truncated death domain; Tumor necrosis factor receptor superfamily member 10D precursor; TR10D_HUMAN; Recombinant human TNFRSF10D protein, C-His-Avi (HEK293) / Biotin |
| 克 隆 号 | |
| 理论分子量 | 17 kDa |
| 检测分子量 | Due to glycosylation, the protein migrates to 38-45 kDa based on Tris-Bis PAGE result. |
| 浓 度 | ＞0.5 mg/ml |
| 纯 度 | >95% as determined by Tris-Bis PAGE; >95% as determined by SEC-HPLC |
| 缓 冲 液 | Lyophilized from 0.22um filtered solution in PBS (pH7.4) with 5mM DTT. Normally 5% trehalose is added as protectant before Lyophilization. |
| 保存条件 | Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L, a member of the TNF family, induces apoptosis of a variety of tumor cell lines. DR4 and DR5 are functional receptors for TRAIL, and DcR1/TRID is a decoy receptor. Another member of the TRAIL receptor family was identified and designated DcR2. The DcR2 receptor is 386 amino acids in length and has an extracellular TRAIL binding domain, but lacks intracellular death domain and does not induce apoptosis. Although this receptor binds to the cytotoxic ligand TRAIL, it contains a truncated death domain and functions as an inhibitory receptor. When overexpressed, the DcR2 receptor can protect cells against TRAIL mediated cytotoxicity. Like DR4 and DR5, DcR2 transcript is widely expressed in a variety of normal human tissues but DcR2 is absent in most tumors. Ultraviolet radiation has been shown to upregulate DcR2 expression on human keratinocytes. Over expression of DcR2 attenuated TRAIL induced apoptosis. DcR1因缺乏细胞内信号传导系统而不能传递凋亡信号,但能竞争性与TRAIL（肿瘤坏死因子相关凋亡诱导配体）结合,有干扰凋亡信号的传导作用。 |
| 产品图片 |
The purity of Biotinylated Human TRAIL R4 / TNFRSF10D Protein is greater than 95% as determined by SEC-HPLC.
Biotinylated Recombinant Human TRAIL R4 / TNFRSF10D Protein on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
