| 产品编号 | bs-47223P-Bio |
| 英文名称 | Recombinant human TSLP protein (R127A, R130A), C-His-Avi (HEK293), Biotin conjugated |
| 中文名称 | 生物素标记重组人TSLP(R127A, R130A)蛋白 |
| 别 名 | Thymic stromal lymphopoietin; Thymic stromal lymphopoietin isoform 1; Thymic stromal lymphopoietin protein TSLP; TSLP protein; TSLP_HUMAN; Recombinant human TSLP protein (R127A, R130A), C-His-Avi (HEK293) / Biotin |
| 克 隆 号 | |
| 理论分子量 | 37 kDa |
| 检测分子量 | Due to glycosylation, the protein migrates to 25-38 kDa based on Tris-Bis PAGE result. |
| 浓 度 | ＞0.5 mg/ml |
| 纯 度 | >95% as determined by Tris-Bis PAGE; >95% as determined by SEC-HPLC |
| 缓 冲 液 | Lyophilized from 0.22um filtered solution in PBS (pH7.4) with 5mM DTT. Normally 5% trehalose is added as protectant before Lyophilization. |
| 保存条件 | Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
Thymic stromal lymphopoietin (TSLP) is a novel cytokine that was found to promote the development of murine B cells in vitro. This protein, which is expressed in a number of tissues including heart, liver and prostate, prevented apoptosis and stimulated growth of the human acute myeloid leukemia (AML)-derived cell line MUTZ-3. Anti-interleukin (IL)-7 receptor antibodies (Abs) neutralized this effect indicating that TSLP binds to at least part of the IL-7 receptor complex. TSLP induced phosphorylation of signal transducer and activator of transcription (STAT)-5. 胸腺基质淋巴细胞生成素-1（胸腺基质细胞分泌的淋巴细胞生成素-1） 为一种新型促进生长的细胞因子——胸腺基质淋巴蛋白（TSLP）可以引发炎症反应， 这种炎症反应可以引起很多过敏性疾病和哮喘。 TSLP可能是过敏性疾病最重要的引发剂，已得到科学家的重视，如果证实了 TSLP是一种起始因子，抑制TSLP可以有效地治疗哮喘和其它过敏性疾病。 |
| 产品图片 |
Immobilized Biotinylated Human TSLP(R127A,R130A) at 0.5μg/ml (100μl/Well). Dose response curve for Anti-Human TSLP Ab.with the EC50 of 61.3ng/ml determined by ELISA.
Immobilized Biotinylated Human TSLP(R127A, R130A),His Tag at 1μg/ml (100μl/Well). Dose response curve for Human TSLPR,hFc Tag with the EC50 of 17.5ng/ml determined by ELISA.
The purity of Biotinylated Human TSLP(R127A, R130A) is greater than 95% as determined by SEC-HPLC.
Biotinylated Human TSLP(R127A, R130A) on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
