产品编号 | bs-40296G-IRDye800CW |
英文名称 | AffiniPure Goat Anti-Mouse IgG H&L(min X Hu, Bov, Hrs, Rb, Sw Sr Prot), IRDye 800CW conjugated |
中文名称 | IRDye 800cw标记的亲和纯化山羊抗小鼠IgG (H+L)(最小交叉反应:Hu, Bov, Hrs, Rb, Sw Sr Prot) |
别 名 | IRDye® 800CW Goat anti-Mouse IgG H+L; 山羊抗小鼠IgG H&L (IRDye® 800CW)预吸附二抗; |
抗体来源 | Goat |
克隆类型 | Polyclonal |
交叉反应 | Mouse |
产品应用 | WB=1:10000-25000,IF=1:200-1200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | kDa |
检测分子量 | |
性 状 | Liquid |
浓 度 | 1.0 mg/ml |
免 疫 原 | Native Mouse IgG |
亚 型 | IgG1 |
纯化方法 | affinity purified by Protein G |
缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol |
保存条件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
产品介绍 | |
产品图片 |
Sample:
Lane 1: Rat Cerebrum tissue lysates
Lane 2: Rat Heart tissue lysates
Lane 3: Human A549 cell lysates
Lane 4: Human Jurkat cell lysates
Lane 5: Human Huvec cell lysates
Lane 6: Human Hela cell lysates
Lane 7: Human U2os cell lysates
Lane 8: Human HepG2 cell lysates
Primary: Anti- GAPDH (bsm-33033M) at 1/50000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG (bs-40296G-IRDye8)at 1/20000 dilution
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |