| 产品编号 | bs-60296G-BF488 |
| 英文名称 | AffiniPure F(ab')₂ Fragment Goat Anti-Mouse IgG (H+L), BF488 conjugated |
| 中文名称 | BF488标记的亲和纯化F(ab)2片段羊抗小鼠IgG H&L |
| 抗体来源 | Goat |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse |
| 产品应用 | IF=1:100-500,Flow-Cyt=1:100-1000,ICC/IF=1:100-1000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | kDa |
| 检测分子量 | |
| 免 疫 原 | Native Mouse IgG |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M PBS (pH7.4). |
| 保存条件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 产品介绍 | |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Vimentin Monoclonal Antibody, Unconjugated (bsm-33170M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (Green,bs-60296G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Vimentin Monoclonal Antibody, Unconjugated (bsm-33170M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (Green,bs-60296G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Vimentin Monoclonal Antibody, Unconjugated (bsm-33170M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (Green,bs-60296G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
