| 产品编号 | bsm-33035R-HRP |
| 英文名称 | PCNA Recombinant Rabbit mAb, HRP conjugated |
| 中文名称 | HRP标记的增殖细胞核抗原（内参）重组兔单抗 |
| 别 名 | Cyclin; DNA polymerase delta auxiliary protein; HGCN8729; MGC8367; Mutagen-sensitive 209 protein; Pcna/cyclin; PCNAR; Polymerase delta accessory protein; Proliferating Cell Nuclear Antigen |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat |
| 产品应用 | IHC-P=1:200-1000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PCNA Recombinant Rabbit mAb, HRP conjugated |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Proliferating cell nuclear antigen (PCNA) is a 28kDa nuclear protein associated with the cell cycle, a nuclear protein vital for cellular DNA synthesis. Proliferating cell nuclear antigen was originally identified by immunofluorescence as a nuclear protein whose appearance correlated with the proliferate state of the cell. PCNA is required for replication of DNA in vitro and has been identified as the auxiliary protein (cofactor) for DNA polymerase delta. The anti-PCNA antibodies react with the nuclei of proliferating cells. PCNA is essential for cellular DNA synthesis and is also required for the in vitro replication of simian virus 40 (SV40) DNA where it acts to coordinate leading and lagging strand synthesis at the replication fork. The PCNA protein may fulfil several separate roles in the cell nucleus associated with changes in its antigenic structure. |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with PCNA monoclonal antibody, HRP conjugated (bsm-33035R-HRP) at 1:1000 dilution and 4°C overnight incubation.
Paraformaldehyde-fixed, paraffin embedded Human Duodenum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PCNA Monoclonal Antibody (bsm-33035R-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PCNA Monoclonal Antibody (bsm-33035R-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PCNA Monoclonal Antibody (bsm-33035R-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PCNA Monoclonal Antibody (bsm-33035R-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PCNA Monoclonal Antibody (bsm-33035R-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PCNA Monoclonal Antibody (bsm-33035R-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
