| 产品编号 | bsm-63104R |
| 英文名称 | phospho-DNA PKcs (S2056) Recombinant Rabbit mAb |
| 中文名称 | 磷酸化DNA依赖性蛋白激酶重组兔单抗 |
| 别 名 | DNA- PKcs; DNA-dependent protein kinase catalytic subunit; DNPK1; EC 2.7.11.1; P460; PRKD; PRKDC; XRCC7; kinase DNA-PK. |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | 8A8 |
| 交叉反应 | Human |
| 产品应用 | WB=1:1000-2000,IHC-P=1:100-200,IHC-F=1:100-200,IF=1:100-200,ICC/IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 469 kDa |
| 检测分子量 | 469 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human DNA PKcs around the phosphorylation site of S2056: YS-pS-QD |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. SWISS: P78527 Gene ID: 5591 DNA依赖的蛋白激酶(DNA-PK)是一种DNA活化的核丝氨酸苏氨酸蛋白激酶. 参与体内DNA双链断裂缺口的修复, 在肿瘤的预后、发生、发展、诊断和治疗等研究中引起科研人员越来越多的重视。 |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-DNA PKcs (S2056) Monoclonal Antibody, Unconjugated (bsm-63104R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
