| 产品编号 | bsm-63162R |
| 英文名称 | IRS1 Recombinant Rabbit mAb |
| 中文名称 | 胰岛素受体底物-1重组兔单抗 |
| 别 名 | HIRS1; Insulin receptor substrate 1; IRS 1; IRS1. |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | 5B3 |
| 交叉反应 | Human |
| 产品应用 | WB=1:1000-2000,IHC-P=1:100-200,IHC-F=1:100-200,IF=1:50-200,ICC/IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 132 kDa |
| 检测分子量 | 170 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human IRS 1: 1100-1200 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
IRS-1 is an adaptor protein that is one of the major substrates of the insulin receptor kinase. Contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain-containing proteins including phosphatidylinositol 3-kinase p85 subunit and GRB-2. SWISS: P35568 Gene ID: 3667 信号传导(Signaling Intermediates) 胰岛素受体底物-1是细胞中分子量为165-185kDa的磷蛋白,胰岛素受体的内源性底物。经胰岛素刺激后其酪氨酸残基被磷酸化。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |