| 产品编号 | bsm-63256R |
| 英文名称 | Villin Recombinant Rabbit mAb |
| 中文名称 | 绒毛蛋白重组兔单抗 |
| 别 名 | VIL; VIL1; Villin1. |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | 5A12 |
| 交叉反应 | Human |
| 产品应用 | WB=1:1000-2000,IHC-P=1:100-200,IHC-F=1:100-200,IF=1:100-200,IP=1:20-50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 93 kDa |
| 检测分子量 | 80 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human Villin 1: 640-693/827 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Epithelial cell-specific Ca2+-regulated actin-modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA); binds LPA with higher affinity than PIP2. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities. SWISS: P09327 Gene ID: 7429 绒毛蛋白(Villin)是一种分子量为93-95kDa的肌动蛋白相关钙调节蛋白,正常分布于肠上皮和肾近曲小管上皮,可用于胃肠道神经内分泌肿瘤诊断的参考指标。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |