| 产品编号 | bs-41403P |
| 英文名称 | Tags Positive Control Whole Cell Lysate, C-His |
| 中文名称 | 标签阳性对照 (18 Tags, 51kDa) 全细胞裂解液 |
| 别 名 | Tags Positive Control (GST; TAP; SRT; AU1; AU5; Avi; CBP; E2; KT3; Nano; S1; Strep-Tag II; VP16; B-tag; RFP; Universal; 100-tag; His); E. coli Positive Control (Escherichia coli) Whole Cell Lysate; E. coli Tags Positive Control (Escherichia coli) Whole Cell Lysate; E.coli Tags Positive Control Whole Cell Lysate; Escherichiacoli Tags Positive Control Whole Cell Lysate; epitope tags; E.coli Tags Positive Control Whole Cell Lysate; Tags Positive Control Whole Cell Lysate, C-His 重组标签蛋白 (18 Tags, 51kDa) ; 大肠杆菌多标签阳性对照裂解液; 大肠杆菌多标签阳性对照全细胞裂解液; 大肠杆菌18 Tags (51kDa) 阳性对照全细胞裂解液; |
| 克 隆 号 | |
| 理论分子量 | 51 kDa |
| 检测分子量 | 51 |
| 性 状 | Liquid |
| 浓 度 | ＞0.5 mg/ml |
| 物 种 | N/A |
| 序 列 | GST tag & TAP tag & SRT tag & AU1 tag & AU5 tag & Avi tag & CBP tag & E2 tag & KT3 tag & Nano-tag & S1-Tag & Strep-Tag II & Strep-Tag II & VP16 tag & B-tag & RFP-tag & Universal-tag & 100-tag & His tag |
| 纯 度 | >95% as determined by SDS-PAGE |
| 纯化方法 | AC |
| 内毒素 | Not analyzed |
| 表达系统 | E.coli |
| 活性 | Not tested |
| 标签 | C-His |
| 缓 冲 液 | 10mM TBS (pH7.4) with 0.02% Proclin300. |
| 保存条件 | Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
This E.coli lysate contains a 51 kd recombinant protein expressing 18 commonly used different epitope tags which are fused to the N-terminus of the protein in the order of: GST tag TAP tag SRT tag AU1 tag AU5 tag Avi tag CBP tag E2 tag KT3 tag Nano-tag S1-Tag Strep-Tag II VP16 tag B-tag RFP-tag Universal-tag 100-tag His tag Lyophilised: After the lyophilised pellet is dissolved in 250 ul SDS-gel sample buffer (1 mg/ml protein lysate) it can be used direct for sample loading and then stored at –20°C. The sample can be reused several times. Excellent as a loading control for Western blotting. Lysate expresses 18 different epitope tags. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
