| 产品编号 | bsm-52837R |
| 英文名称 | CD147 |
| 中文名称 | CD147重组兔单抗 |
| 别 名 | TCSF; Emmprin; 5A11 antigen; 5F7; Basigin (Ok blood group); Basigin; Blood brain barrier HT7 antigen; BSG; CD 147; CD147 antigen; Collagenase stimulatory factor; Extracellular matrix metalloproteinase inducer; Leukocyte activation antigen M6; M6; M6 leukocyte activation antigen; neurothelin; OK; OK blood group; OK blood group antigen; TCSF; Tumor cell derived collagenase stimulatory factor; BASI_HUMAN. 细胞外基质金属蛋白酶诱导因子抗体 |
 |
Specific References (1) | bsm-52837R has been referenced in 1 publications.
[IF=2.934] Xuejiao Zhu. et al. Basigin-CyP elevated porcine circovirus type2 replication. Virus Res. 2020 Nov;289:198152
|
| 研究领域 | 肿瘤 免疫学 合成与降解 细胞表面分子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | 12C3 |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | WB=1:1000-2000, IHC-P=1:100-500, IHC-F=1:50-200, ICC=1:50-200, IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 42kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant human CD147 protein |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. The encoded protein is also a member of the immunoglobulin superfamily. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Function: Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes. Subunit: Forms homooligomers in a cis-dependent manner on the plasma membrane. Forms heterooligomers of isoform 2 and isoform 3. Forms a complex with MMP1 at the tumor cell surface. Interacts with SLC16A1 and SLC1A3; probably a BSG dimer is associated with a monocarboxylate transporter dimer. Interacts with ATP1B2, MAG and L1CAM. Interacts with AJAP1. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Melanosome. Tissue Specificity: Present only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas. Post-translational modifications: N-glycosylated. Similarity: Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: P35613 Gene ID: 682 Database links: Entrez Gene: 682 Human Entrez Gene: 12215 Mouse Omim: 109480 Human SwissProt: P35613 Human SwissProt: P18572 Mouse Unigene: 501293 Human |
| 产品图片 |
Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 ug Lane 2: Liver (Mouse) Lysate at 40 ug Lane 3: Eye (Mouse) Lysate at 40 ug Lane 4: Cerebrum (Rat) Lysate at 40 ug Lane 5: Liver (Rat) Lysate at 40 ug Lane 6: HepG2 (Human) Cell Lysate at 30 ug Lane 7: MCF-7 (Human) Cell Lysate at 30 ug Primary: Anti-CD147 (bsm-52837R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40-70 kD Observed band size: 58 kD |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
