| 产品编号 | bsm-51112M |
| 英文名称 | TLE1 |
| 中文名称 | 转导样增强蛋白1小鼠单克隆抗体 |
| 别 名 | TLE 1; E(Sp1) homolog; Enhancer of split groucho 1; Enhancer of split groucho like protein 1; Enhancer of split groucho-like protein 1; ESG; ESG1; GRG1; TLE1; TLE1_HUMAN; Transducin like enhancer of split 1 (E(sp1) homolog Drosophila); Transducin like enhancer of split 1; Transducin like enhancer of split 1 homolog of Drosophila E(sp1); Transducin like enhancer protein 1; Transducin-like enhancer protein 1. |
| 研究领域 | 细胞生物 信号转导 干细胞 转录调节因子 表观遗传学 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | 1C1 |
| 交叉反应 | (predicted: Human) |
| 产品应用 | WB=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 83kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TLE1 |
| 亚 型 | IgG1 |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
TLE1 (Transducin Like Enhancer Of Split 1) is a Protein Coding gene. Diseases associated with TLE1 include Synovium Cancer and Spindle Cell Liposarcoma. Among its related pathways are Signaling by Wnt and Wnt / Hedgehog / Notch. Gene Ontology (GO) annotations related to this gene include identical protein binding and transcription factor binding. An important paralog of this gene is TLE4. Function: Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG. Subunit: Homooligomer and heterooligomer with other family members. Subcellular Location: Nucleus. Nuclear and chromatin-associated, depending on isoforms and phosphorylation status. Hyperphosphorylation decreases the affinity for nuclear components. Tissue Specificity: In all tissues examined, mostly in brain, liver and muscle. Post-translational modifications: Phosphorylated, probably by CDK1. The degree of phosphorylation varies throughout the cell cycle, and is highest at the G2/M transition. Becomes hyperphosphorylated in response to cell differentiation and interaction with HES1 or RUNX1. Ubiquitinated by XIAP/BIRC4. Similarity: Belongs to the WD repeat Groucho/TLE family. Contains 6 WD repeats. SWISS: Q04724 Gene ID: 7088 Database links: Entrez Gene: 7088 Human Entrez Gene: 21885 Mouse Omim: 600189 Human SwissProt: Q04724 Human SwissProt: Q62440 Mouse Unigene: 197320 Human Unigene: 689805 Human Unigene: 278444 Mouse Unigene: 388819 Mouse Unigene: 399716 Mouse Unigene: 6875 Rat |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
