| 产品编号 | bsm-54469R |
| 英文名称 | Fatty Acid Synthase |
| 中文名称 | 脂肪酸合成酶重组兔单抗 |
| 别 名 | fatty acid synthase;FAS; FASN; FA Synthase 1; MGC14367; MGC15706; OA-519; Fatty acid synthase; [Acyl-carrier-protein] S-acetyltransferase; [Acyl-carrier-protein] S-malonyltransferase; 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase; 3-oxoacyl-[acyl-carrier-protein] reductase; 3-oxoacyl-[acyl-carrier-protein] synthase; Enoyl-[acyl-carrier-protein] reductase; Oleoyl-[acyl-carrier-protein] hydrolase; FAS_HUMAN. |
| 研究领域 | 肿瘤 心血管 免疫学 神经生物学 信号转导 激酶和磷酸酶 合成与降解 |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | 9B6 |
| 交叉反应 | Human (predicted: Mouse,Rat) |
| 产品应用 | WB=1:500-1000, IHC-P=1:50-200, IHC-F=1:50-200, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 273kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant human Fatty Acid Synthase |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
FASN (Fatty Acid Synthase) is a central enzyme in de novo lipogenesis. FAS is a target for SREBP and is upregulated by LXR activation; it is also one of the accepted markers for insulin resistance, SREBP and LXR activation. Function: Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein. Subunit: Homodimer which is arranged in a head to tail fashion. Subcellular Location: Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Tissue Specificity: Ubiquitous. Prominent expression in brain, lung, and liver. Similarity: Contains 1 acyl carrier domain. SWISS: P49327 Gene ID: 2194 Database links: Entrez Gene: 2194 Human Entrez Gene: 14104 Mouse Omim: 600212 Human SwissProt: P12276 Chicken SwissProt: P49327 Human SwissProt: P19096 Mouse Unigene: 8951 Chicken Unigene: 30099 Cow Unigene: 83190 Human Unigene: 236443 Mouse Unigene: 9486 Rat 合成与降解（Synthesis and Degradation） 脂肪酸合成酶fatty acid synthase是一种新的肿瘤标志物,高表达常提示肿瘤的预后不良. |
| 产品图片 |
Sample:
Lane 1: Human A549 cell lysates Lane 2: Human MCF-7 cell lysates Primary: Anti-Fatty Acid Synthase (bsm-54469R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 273 kDa Observed band size: 250 kDa 
Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by microwaving in EDTA buffer (Ph8.0) for 5min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Fatty Acid Synthase) Monoclonal Antibody, Unconjugated (bsm-54469R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human breast); Antigen retrieval by microwaving in EDTA buffer (Ph8.0) for 5min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Fatty Acid Synthase) Monoclonal Antibody, Unconjugated (bsm-54469R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
