| 产品编号 | bs-55138R |
| 英文名称 | [KO验证抗体] N-Cadherin |
| 中文名称 | N-钙粘附分子抗体 |
| 别 名 | Cadherin 2; Cadherin 2 N cadherin neuronal; Cadherin 2 type 1; Cadherin 2 type 1 N cadherin neuronal; cadherin 2 type 1 N-cadherin neuronal; Cadherin2; Calcium dependent adhesion protein neuronal; CD325; CD325 antigen; CDH2; CDHN; CDw325; CDw325 antigen; N cadherin 1; NCAD; Neural Cadherin; Neural Cadherin. |
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Specific References (1) | bs-55138R has been referenced in 1 publications.
[IF=3.388] Liu H et al. Circular RNA has_circ_0003204 inhibits oxLDL-induced vascular endothelial cell proliferation and angiogenesis. Cell Signal. 2020 Jun;70:109595. WB ; human.
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| 研究领域 | 肿瘤 细胞生物 免疫学 激酶和磷酸酶 细胞粘附分子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse (predicted: Rat) |
| 产品应用 | WB=1:500-1000, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 97/99kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human N-Cadherin: 750-850-905 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product. Function: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. Subcellular Location: Cell membrane. Similarity: Contains 5 cadherin domains. SWISS: P19022 Gene ID: 1000 Database links: Entrez Gene: 1000 Human Entrez Gene: 12558 Mouse SwissProt: P19022 Human SwissProt: P15116 Mouse 细胞粘附蛋白（Call Adhesion Protein） N-钙粘附分子属于依赖钙离子的细胞间糖蛋白粘附分子的一部分,主要是参与细胞间同源性粘附,调控和介导细胞间相互反应。 |
| 产品图片 |
Sample:
Lane 1: Hela (Human) Cell Lysate at 25 ug Lane 2: N-Cadherin knockout (KO) HeLa (Human) Cell Lysate at 25 ug Primary: Anti-N-Cadherin (bs-55138R) at 1/1000 dilution Secondary: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution Predicted band size: 130 kD Observed band size: 135 kD 
Sample:
Lane 1: Heart (Mouse) Lysate at 40 ug Lane 2: A549 (Human) Cell Lysate at 30 ug Primary: Anti-N-Cadherin (bs-55138R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 130 kD Observed band size: 130 kD |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
