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[KO验证抗体] Rabbit Anti-RHOC  antibody (bs-55187R)
~~~促销,代码SQ220725~~~
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说明书: 50ul  100ul  200ul
50ul/1138.00元
100ul/1880.00元
200ul/2900.00元
大包装/询价

产品编号 bs-55187R
英文名称 [KO验证抗体] RHOC
中文名称 RhoC抗体
别    名 ARH 9; ARH9; ARHC; H9; MGC1448; MGC61427; Oncogene RHO H9; Ras homolog gene family member C; RAS related homolog 9; RHO C; Rho cDNA clone 9; Rho related GTP binding protein RhoC; Rho-related GTP-binding protein RhoC; rhoC; RhoC GTPase; RHOC_HUMAN; RHOH 9; RHOH9; Small GTP binding protein RhoC.  
研究领域 肿瘤  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse,  (predicted: Rat, )
产品应用 WB=1:500-2000 IHC-P=1:50-100 IHC-F=1:450-100 IF=1:50-100 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 22kDa
细胞定位 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 Recombinant human RHOC: 1-193/193 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]

Function:
Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers.

Subunit:
Interacts with RTKN. Interacts with AKAP13, DIAPH1, PKN2, ROCK1 and ROCK2. Interacts with ARHGDIA.

Subcellular Location:
Cell membrane; Lipid-anchor; Cytoplasmic side (Potential). Cleavage furrow. Note=Translocates to the equatorial region before furrow formation in a ECT2-dependent manner.

Similarity:
Belongs to the small GTPase superfamily. Rho family.

SWISS:
P08134

Gene ID:
389

Database links:

Entrez Gene: 389 Human

Entrez Gene: 295342 Rat

Omim: 165380 Human

SwissProt: P08134 Human

Unigene: 502659 Human

Unigene: 658289 Human



产品图片
Sample:
Lane 1: 293T (Human) Cell Lysate at 25 ug
Lane 2: RHOC knockout (KO) 293T (Human) Cell Lysate at 25 ug
Primary: Anti-RHOC (bs-55187R) at 1/1000 dilution
Secondary: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution
Predicted band size: 22 kD
Observed band size: 22 kD
Sample:
Lane 1: NIH/3T3 (Mouse) Cell Lysate at 30 ug
Lane 2: U2os (Human) Cell Lysate at 30 ug
Lane 3: Huvec (Human) Cell Lysate at 30 ug
Lane 4: A431 (Human) Cell Lysate at 30 ug
Lane 5: U251 (Human) Cell Lysate at 30 ug
Primary:
Anti- RHOC (bs-55187R) at 1/1000 dilution
Anti-beta-Actin (bs-0061R) at 1/2000 dilution
Secondary:
IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 22 kD
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (KO Validated)RHOC polyclonal Antibody, Unconjugated (bs-55187R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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