| 产品编号 | bs-55211R |
| 英文名称 | [KO验证抗体] TAGLN2 |
| 中文名称 | 细胞骨架相关蛋白2抗体 |
| 别 名 | CDABP0035; epididymis tissue protein Li 7e; HA1756; KIAA0120; SM22-alpha homolog; TAGL2_HUMAN; Tagln2; Transgelin 2; Transgelin-2. |
| 研究领域 | 肿瘤 细胞生物 肿瘤细胞生物标志物 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse |
| 产品应用 | WB=1:500-2000, ICC=1:100, IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 22kDa |
| 细胞定位 | 细胞浆 细胞外基质 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant human TAGLN2: 1-199/199 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is similar to the protein transgelin, which is one of the earliest markers of differentiated smooth muscle. The specific function of this protein has not yet been determined, although it is thought to be a tumor suppressor. Multiple transcript variants encoding different isoforms have been found for this gene. Similarity: Belongs to the calponin family. Contains 1 calponin-like repeat. Contains 1 CH (calponin-homology) domain. SWISS: P37802 Gene ID: 8407 Database links: Entrez Gene: 8407 Human Omim: 604634 Human SwissProt: P37802 Human Unigene: 517168 Human |
| 产品图片 |
Sample:
Lane 1: 293T (Human) Cell Lysate at 25 ug Lane 2: TAGLN2 knockout (KO) 293T (Human) Cell Lysate at 25 ug Primary: Anti-TAGLN2 (bs-55211R) at 1/1000 dilution Secondary: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution Predicted band size: 18 kD Observed band size: 20 kD 
Sample:
Lane 1: Lung (Mouse) Lysate at 40 ug Lane 2: Placenta (Mouse) Lysate at 40 ug Lane 3: Uterus (Mouse) Lysate at 40 ug Lane 4: U251 (Human) Cell Lysate at 30 ug Lane 5: A431 (Human) Cell Lysate at 30 ug Lane 6: U87MG (Human) Cell Lysate at 30 ug Lane 7: U2os (Human) Cell Lysate at 30 ug Lane 8: Huvec (Human) Cell Lysate at 30 ug Lane 9: A673 (Human) Cell Lysate at 30 ug Primary: Anti-TAGLN2 (bs-55211R) at 1/1000 dilution Anti-beta-Actin (bs-0061R) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18 kD Observed band size: 22 kD 
MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (KO Validated)TAGLN2 polyclonal Antibody, Unconjugated (bs-55211R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
