| 产品编号 | bsm-34028M |
| 英文名称 | ADGRE1 (F4/80) |
| 中文名称 | 小鼠抗表皮生长因子样激素受体1（EMR1）单克隆抗体 |
| 别 名 | F4/80 (mouse); Adhesion G protein-coupled receptor E1; Cell surface glycoprotein EMR1; Cell surface glycoprotein F4/80; DD7A5 7; Egf like module containing mucin like hormone receptor like 1; Egf like module containing mucin like hormone receptor like sequence 1; EGF like module receptor 1; EGF TM7; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EGFTM7; EMR 1; EMR1; EMR-1; EMR1 hormone receptor; EMR1_HUMAN; AGRE1_HUMAN; Gpf480; Ly71; Lymphocyte antigen 71; TM7LN3. |
 |
Specific References (8) | bsm-34028M has been referenced in 8 publications.
|
| 研究领域 | 细胞生物 免疫学 生长因子和激素 G蛋白偶联受体 糖蛋白 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | 3D4 |
| 交叉反应 | Human (predicted: Rat,Mouse) |
| 产品应用 | WB=1:100-1000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 95kDa |
| 检测分子量 | 160 kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 200ug/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse F4/80 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M PBS(pH7.4) with 0.1% BSA, 0.03% Proclin300. |
| 保存条件 | Shipped at 4℃. Store at 2-8℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The epidermal growth factor (EGF)-TM7 family constitutes a group of class B G-protein coupled receptors, which includes CD97, EMR1 (EGF-like molecule containing mucin-like hormone receptor 1, designated F4/80 in mouse), EMR2, EMR3, FIRE, and ETL (1–3). These family members are characterized by an extended extracellular region with several N-terminal EGF domains, and are predominantly expressed on cells of the immune system (1–3). The EGF-TM7 protein family are encoded by a gene cluster on human chromosome 19p13 (1,3,4). The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria, and Langerhans cells in the skin (1). F4/80/EMR1 undergoes extensive N-linked glycosylation as well as some O-linked glycosylation (5,6). The function of F4/80/EMR1 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration, or as a G-protein coupled signaling component of macrophages. Function: Could be involved in cell-cell interactions. Subunit: Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. Contains 6 EGF-like domains. Contains 1 GPS domain. Subcellular Location: Cell membrane. Tissue Specificity: Wide expression; increased levels in peripheral blood mononuclear cells. Similarity: Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. Contains 6 EGF-like domains. Contains 1 GPS domain. SWISS: Q14246 Gene ID: 2015 Database links: Entrez Gene: 2015 Human Entrez Gene: 13733 Mouse Omim: 600493 Human SwissProt: Q14246 Human SwissProt: Q61549 Mouse Unigene: 2375 Human Unigene: 2254 Mouse |
| 产品图片 |
Sample:
Lane 1: Human HL-60 cell lysates Primary: Anti-ADGRE1 (F4/80) (bsm-34028M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 95 kDa Observed band size: 120 kDa |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
