| 产品编号 | bs-104969P |
| 英文名称 | Recombinant Human P53 Protein, N-His |
| 中文名称 | |
| 别 名 | P53_HUMAN; Cellular tumor antigen p53; TP53; Antigen NY-CO-13;Phosphoprotein p53; Tumor suppressor p53; tumor protein p53; BCC7; LFS1; BMFS5; TRP53; P 53; P-53; wt-p53; Widespread p53; Wtp53; Wt p53; |
| 克 隆 号 | |
| 理论分子量 | 26.85 kDa |
| 性 状 | Lyophilized |
| 纯 度 | >90% as determined by SDS-PAGE. |
| 缓 冲 液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 保存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons (PMIDs: 12032546, 20937277). [provided by RefSeq, Feb 2013]. SWISS: P04637 Gene ID: 7157 wtp53广泛的研究发现P53肿瘤抑制基因对50%以上的人类癌症具有抑制突变的功能。P53蛋白水平在正常细胞中表达低，在DNA突变时或各种各样细胞遇难信号时反应增加。该基因突变或缺失是导致许多肿瘤发生的原因。 野生型P53(wt-p53)可诱导细胞凋亡，并通过细胞凋亡抑制肿瘤生长，而P53的突变或缺失则可抑制野生型P53的功能，使得缺陷细胞得以存活下来，从而导致肿瘤发生。 P53同时也是细胞凋亡的调控因子。此抗体可用于P53肿瘤抑制基因功能的研究。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
