| 产品编号 | bs-105223P |
| 英文名称 | Recombinant Human FFAR1 Protein, N-His-KSI |
| 中文名称 | |
| 别 名 | Free fatty acid receptor 1; G-protein coupled receptor 40; FFAR1; GPR40 |
| 克 隆 号 | |
| 理论分子量 | 25.96 kDa |
| 性 状 | Lyophilized |
| 纯 度 | >90% as determined by SDS-PAGE. |
| 缓 冲 液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 保存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
G protein coupled receptors provide attractive targets for drug therapy due to the sheer size and diversity of ligands within this receptor family. G protein-coupled receptor 40 (GPR40) functions as a cell-surface receptor for long-chain free fatty acids (FFAs). FFAs provide an important energy source, but also function as signaling molecules in various pathways, notably the process of insulin secretion. In pancreatic tissue, the interaction of long chain FFAs with GPR40 amplifies glucose-stimulated insulin secretion from beta cells, suggesting a possible role for GPR40 in the treatment of diabetes associated with insulin-deficiency. Specifically, the Arg211His polymorphism in the GPR40 gene may contribute to the variation of insulin secretory capacity in Japanese men. Also, GPR40 may be involved in the control of breast cancer cell growth by fatty acids and, therefore, provide a link between fat and cancer. SWISS: O14842 Gene ID: 2864 FFAR1 (GPR40)实际分子量约为31 kDa. (J. Biol. Chem. (2018) 293(27) 10675–10691; J Alzheimers Dis Parkinsonism 2018, 8:6; SCIENtIfIC Reports, (2018) 8:14318; Sci. Adv. 2018; 4 : eaau2357)(Jim 2020-05-21) |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
