| 产品编号 | bs-105295P |
| 英文名称 | Recombinant Human N4BP1 Protein, N-His |
| 中文名称 | |
| 别 名 | NEDD4-binding protein 1; N4BP1; 3.1.-.-; N4BP1; KIAA0615 |
| 克 隆 号 | |
| 理论分子量 | 23.42 kDa |
| 性 状 | Lyophilized |
| 纯 度 | >90% as determined by SDS-PAGE. |
| 缓 冲 液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 保存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
N4BP1 is an 896 amino acid protein that belongs to the N4BP1 family. N4BP1 primarily localizes to nucleolus, however it also localizes to PML nuclear bodies when desumoylated. Detected in heart, lung, brain, liver, skeletal muscle, pancreas, kidney, spleen, testis and ovary, N4BP1 is phosphorylated upon DNA damage probably by ATM or ATR. N4BP1 interacts with Nedd4, a protein with a wide range of activities including modification of ubiquitin-protein ligase, regulation of membrane channels and potentiating hormone-dependent activation of transcription. N4BP1 inhibits the E3 ubiquitin-protein ligase ITCH, by interacting with the second WW domain of ITCH thereby competing with ITCH's substrates and impairing ubiquitination of substrates. N4BP1 has no obvious transmembrane regions, which raises the possibility that N4BP1 represents a soluble adaptor that could sequester ITCH within the cytosol. The N4BP1 gene maps to chromosome 16q12.1. SWISS: O75113 Gene ID: 9683 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
